Yes, It is Possible to Perform DNA Profiling with SNPs instead of SSRs as DNA Markers
DNA profiling is a process used to uncover genetic information about a person. It is used in forensic settings to identify people from crime scenes and in medical settings to diagnose genetic disorders. Traditionally, Short Sequence Repeats (SSRs) were used as DNA markers for DNA profiling, but recently, Single Nucleotide Polymorphisms (SNPs) have become more common as markers for DNA profiling. SNPs are preferable to SSRs for several reasons. SNPs are more abundant than SSRs and have a higher degree of polymorphism, meaning they have more variation between individuals (Schlötterer 2018). Additionally, they have better transferability between species and are less prone to mutation (Gauci et al. 2020). This makes them more reliable for DNA profiling purposes and also makes them easier to use in a variety of species.
SNPs can be used in a variety of methods in DNA profiling, such as restriction fragment length polymorphism (RFLP), single-strand conformational polymorphism (SSCP), and polymerase chain reaction (PCR). These methods are more efficient and cost-effective than those used with SSRs (Xu et al. 2018). Additionally, SNPs require less DNA to be analyzed, meaning that DNA profiling can be done with smaller samples (Rost et al. 2021). Cont….
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