Why Might a Sample/ Standard Curve Not Obey Beer’s Law?,Why might a sample/ standard curve not obey Beer’s law?

Why Might a Sample/ Standard Curve Not Obey Beer’s Law?

Beer’s law states that the absorbance of a sample is directly proportional to its concentration. However, in some cases, a sample/standard curve may not obey Beer’s Law. This can be due to a variety of factors including the sample’s purity, the presence of interfering compounds in the sample, or a change in the sample’s pH or temperature. Additionally, the instrument being used to measure the sample’s absorbance may not be properly calibrated, or the sample may not be diluted properly. The purity of the sample is one of the main factors that can lead to a sample/standard curve not following Beer’s Law. Contamination in the sample can interfere with the absorbance measurements, resulting in inaccurate measurements and an inability to follow the linear relationship of Beer’s Law (Guan, 2016; Lan et al., 2021). Additionally, the presence of interfering compounds in the sample can lead to inaccurate measurements. For example, certain compounds such as chromophores, fluorophores, and heavy metals can absorb light, leading to inaccurate measurements (Tiwari et al., 2016; Puspawati et al., 2021). Cont…..

 

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