The Vector of Choice for Subcloning the Kinase Domain of the EGFR Gene: A Review,You would like to study the gene encoding the epidermal growth factor receptor (EGFR), as this gene is mutated in a subset of non-small cell lung cancer patients. You subclone the portion of the gene encoding the kinase domain, a 1.2kb region, for further study. a. State what type of vector you would use and why.

The Vector of Choice for Subcloning the Kinase Domain of the EGFR Gene: A Review

The epidermal growth factor receptor (EGFR) gene is mutated in a subset of non-small cell lung cancer patients, and its kinase domain, a 1.2kb region, is of particular interest for further study. Therefore, it is necessary to select a vector to subclone this region for subsequent analyses. There are several types of vectors available for subcloning, including plasmid, BAC, YAC, and cosmid vectors (Jiang, et al., 2020). Plasmid vectors are the most commonly used for subcloning, as they are readily available, highly efficient, and offer flexibility in the choice of selection markers and cloning sites (Mishra and Prasad, 2016). Plasmid vectors are also relatively small, with a typical size of 5kb, which is well suited for the 1.2kb region of the EGFR kinase domain (Jiang et al., 2020). In conclusion, the vector of choice for subcloning the kinase domain of the EGFR gene is a plasmid vector, due to its efficiency, availability, and size. This vector can be used to successfully subclone the kinase domain for further analysis which may reveal insights into the role of this gene in non-small cell lung cancer. Cont….

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