The Four Ways Light Interacts with Cell Cultures: An Overview,You are performing a light assay to determine how light interacts with your cell culture. What are the four ways the light can interact with your sample?

The Four Ways Light Interacts with Cell Cultures: An Overview

Light can interact with cell cultures in four different ways: absorbance, fluorescence, scattering, and luminescence (Lai et al., 2018). Absorbance measures the amount of light that is blocked or absorbed by the sample. Fluorescence occurs when light is absorbed and then re-emitted at a different wavelength (Chen et al., 2020). Scattering occurs when light is scattered off the surface of the sample. Finally, luminescence is the emission of light from a chemical reaction (Wu et al., 2021). These four ways of light interaction can be used to measure various characteristics of the sample. For example, absorbance can be used to measure the concentration of a molecule in a sample (Lai et al., 2018). Fluorescence can be used to measure the amount of a certain molecule present in a sample (Chen et al., 2020). Scattering and luminescence can be used to measure the size, shape, and other properties of particles in the sample (Wu et al., 2021). Overall, light has the ability to interact with cell cultures in four different ways. Absorbance, fluorescence, scattering, and luminescence are all useful techniques for measuring the characteristics of a sample. Cont…