The Efficiency of Coomassie Brilliant Blue Staining After SDS-PAGE,Which one statement about Coomassie Brilliant Blue staining after SDS-PAGE is incorrect? (mark all that apply) A We use Coomassie Brilliant Blue to stain all proteins in a polyacrylamide gel to prove that protein samples were correctly normalized and loaded evenly. B We use Coomassie Brilliant Blue staining to purify our protein of interest in SDS PAGE

The Efficiency of Coomassie Brilliant Blue Staining After SDS-PAGE

The SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a method of separating proteins according to their size and charge. Coomassie Brilliant Blue (CBB) is a commonly used protein staining method after SDS-PAGE. CBB staining is a simple, reliable, and effective method to visualize the presence and composition of proteins in a sample. The CBB staining technique is rapid, sensitive, and gives a wide range of detection from nanogram to milligram amounts of proteins (Kumar et al., 2016). It is also relatively inexpensive and is compatible with most protein-containing samples for SDS-PAGE (Kumar et al., 2016). CBB staining is a two-step process, which includes staining and destaining of the gel. The staining step is the most important part since it is responsible for the visualization of the proteins present in the sample. The staining solution is composed of Coomassie Brilliant Blue G-250 and 65% methanol. After staining, the proteins can be visualized as blue bands against a clear background (Ramírez-González et al., 2021). Cont…

 

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