The Discovery of Taq Polymerase and its Use in PCR
The discovery of Taq Polymerase, a heat-resistant enzyme, was made in 1976 at the Cetus Corporation by Kary Mullis (Rabilloud, 2017). Taq Polymerase is derived from a bacteria, Thermus aquaticus, found in hot springs near the Yellowstone National Park (Ellington & McPherson, 2016). This discovery revolutionized the field of molecular biology, as the enzyme had the ability to withstand high temperatures and facilitate the process of PCR (Polymerase Chain Reaction) (Kumar, Singh & Sugimoto, 2020). PCR is a technique used in molecular biology that amplifies a single or few copies of a specific DNA segment (Kumar et al., 2020). This process is achieved by using Taq Polymerase to create multiple copies of the target DNA, allowing for the accurate and quick analysis of genetic material (Larimore et al., 2019). This process is used in a variety of fields, from the diagnosis of genetic diseases to the identification of pathogens in clinical settings (Kumar et al., 2020). Cont….
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