The Best Methods of PCR Technology: A Comparison of Southern Blot, Northern Blot, Reverse Transcription PCR, Real-Time, and Gene Microarrays
Polymerase Chain Reaction (PCR) is a powerful tool used in molecular biology to amplify and study DNA. PCR allows for the amplification and analysis of specific segments of DNA so that it can be used to diagnose diseases, identify genetic disorders, and investigate genetic variation. Due to its wide range of applications, there are a variety of PCR techniques available (Kumari et al., 2020). The most commonly used PCR techniques include Southern Blot, Northern Blot, Reverse Transcription PCR, Real-Time PCR, and Gene Microarrays. Each of these techniques has different parameters, such as the amount of DNA needed for analysis, the complexity of the reaction, the speed of the reaction, and the cost. Southern Blot is a technique used to detect and analyze DNA fragments of a particular size. It is often used to detect specific genetic mutations or to investigate an organism’s genome (Justin & Jain, 2022). Cont…
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