Comparing and Contrasting E. coli and Yeast as Host Protein Expression Systems
In biotechnology, protein expression via host organisms is a crucial part of many processes. Two of the most common hosts used for protein expression are Escherichia coli (E. coli) and Saccharomyces cerevisiae (yeast). While both of these organisms can be used effectively for protein expression, they have distinct characteristics that make them better suited for certain applications. E. coli is a prokaryotic organism that is widely used as a host for protein expression due to its ease of handling, low cost, and high yields of the expressed protein. E. coli is also able to synthesize proteins that are difficult for yeast to produce, such as those containing disulfide bonds (Ladner, 2016). Furthermore, E. coli is able to tolerate the presence of detergents, which can be helpful for purification (Kumar et al., 2021). In contrast, yeast is a eukaryotic organism that is typically preferred for the expression of eukaryotic proteins due to its ability to. Cont…
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