Investigating the Role of Transcription Factors in Damaged Fins for YFG Expression, In a damaged fin, a gene called YFG is expressed that leads to repair and regeneration of fin. There is an enhancer region for the YFG gene that is required for expression of YFG completely. We do not know whether the (option 1) enhancer is bound by a transcriptional repressor in undamaged fins that leads to no YFG expression, or whether (option 2) there is a transcriptional activator that binds to enhancer in damaged fins which leads to YFG expression. Design a comprehensive experiment to determine which is the case from above 2 options. What genome analysis techniques will you use for such an experiment and explain the experiment in detail in light of those techniques.

Investigating the Role of Transcription Factors in Damaged Fins for YFG Expression

In order to determine whether a transcriptional repressor or activator is responsible for YFG expression in damaged fins, a comprehensive experiment can be designed that utilizes a combination of genome analysis techniques. The experiment should begin by conducting a transcriptome analysis of both undamaged and damaged fins to identify all transcription factors that are expressed in both conditions. These transcription factors should then be analyzed using a Bioinformatics approach to identify any potential binding sites within the enhancer region of the YFG gene (Kanodia et al., 2019). Once these potential binding sites have been identified, the experiment should then proceed to validate the binding of the transcription factors by conducting a ChIP-seq analysis (Cortes-Ciriano et al., 2018). This technique involves using an antibody to target the transcription factors and measure the enrichment of the enhancer region of the YFG gene in both undamaged and damaged fins. This will allow us to determine whether the transcription factors are binding to the enhancer region in damaged fins, thus leading to YFG expression. Cont….