Analysis of Intracellular Distribution of Giardia IscU and Human Proteins by Cell Fractionation,Alpha-ketoglutarate dehydrogenase (?-KGDH) is an enzyme involved in the citric acid cycle of respiration, and it is regulated in various ways. In the presence of high adenosine monophosphate (AMP), high succinyl coenzyme A (succinyl-CoA), and no external regulators, the resulting allosteric enzyme curves of ?-KGDH can be significantly impacted. Studies have shown that when there are no external regulators, the allosteric enzyme curves of ?-KGDH will show an increase in activity as the substrate concentration increases (Kubota, et al., 2018). This is known as the positive cooperativity effect, and it is primarily caused by the formation of the enzyme-substrate complex and the increased affinity of the enzyme for the substrate (Klussmann, et al., 2017). However, when regulators are present, the allosteric enzyme curves can be significantly altered. For instance, in the presence of AMP, the allosteric enzyme curves of ?-KGDH will show a decrease in activity as the substrate concentration increases (Kubota, et al., 2020). Cont….

Analysis of Intracellular Distribution of Giardia IscU and Human Proteins by Cell Fractionation

Cell fractionation is a technique that is used to separate the components of a cell on the basis of size and density. This technique has been applied to the analysis of the intracellular distributions of the Giardia protein IscU and the human protein. Each cell homogenate was centrifuged at low speed to separate the low-speed sediment (pellet) from the low-speed supernatant. This supernatant was then subjected to high-speed centrifugation to separate the high-speed sediment (pellet) from the cytosol (supernatant). The results of this study have been used to better understand the intracellular localization of these proteins (Kumar et al., 2021; Yi et al., 2016). In the low-speed centrifugation, the pellet is composed of organelles, including the nucleus, mitochondria and other membranous structures, while the supernatant contains mainly cytoplasmic components in solution. In the high-speed centrifugation, the pellet contains mainly membrane-bound organelles, while the supernatant contains mainly cytosolic components. The results of the analysis showed that Giardia IscU is mainly located in the cytosol, while the human protein is mainly located in the membrane-bound organelles (Kumar et al., 2021; Yi et al., 2016). Cont…..