Val-Ala-Ala-His-Glu-X-Gly-His-X-X-Gly-X-X-His In this sequence which amino acid would be deprotonated? What is the pI or this sequence? Which amino acid has a pka ~7.0? Val-Ala-Ala-His-Glu-X-Gly-His-X-X-Gly-X-X-His


The sequence Val-Ala-Ala-His-Glu-X-Gly-His-X-X-Gly-X-X-His accommodates two amino acids with pKa values of roughly 7.0. These two amino acids are Histidine (His) and Glutamic Acid (Glu). For the reason that pKa of Histidine is increased than that of Glutamic Acid, Histidine is the amino acid that will be deprotonated when the answer has a pH of seven.0 or decrease. The pI of this sequence is 6.52, calculated by discovering the imply of the pKa values of the 2 acidic amino acids within the sequence, Glutamic Acid and Histidine. Particularly, the pKa of Glutamic Acid is 4.25 and that of Histidine is 6.0 (Graf, 2016; Navarro et al., 2021). Subsequently, the pI of the Val-Ala-Ala-His-Glu-X-Gly-His-X-X-Gly-X-X-His sequence is 6.52 (4.25 + 6.0)/2. Which means that any answer with a pH increased than 6.52 could have Histidine deprotonated and any answer with a pH decrease than 6.52 could have Glutamic Acid deprotonated. Cont…

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