EcoR1 and CRISPR/Cas9: An In-depth Analysis
Two powerful tools in molecular biology are EcoR1 and CRISPR/Cas9, both used for biotechnology and molecular biology. CRISPR/Cas9 uses a nuclease enzyme for precise genetic modification in many organisms. EcoR1 (restrictive enzyme) is responsible for recognizing and cutting a certain DNA sequence. CRISPR/Cas9 with EcoR1 often work together to alter or modify the DNA code in a cell. CRISPR/Cas9, EcoR1 are capable of modifying a cell’s genome sequence with astonishing accuracy and efficiency, as demonstrated by recent research (Liu, Wang, and colleagues, 2020). CRISPR/Cas9 is able to insert a particular mutation into a gene. EcoR1 allows for the deletion of DNA in specific locations to allow the mutation to be added. It has been used successfully to modify DNA of many organisms including yeast and bacteria (Komor et. al. 2016).
CRISPR/Cas9, EcoR1 and CRISPR/Cas9 have been combined to make it possible to produce designer plasmids (Komor et. al. 2019,). Cont….
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