Describe how the Lineweaver-Burk plot is derived from the Michaelis-Menten equation, and draw examples of the Lineweaver-Burk plots expected in the presence or absence of (a) a competitive reversible inhibitor, and (b) a non-competitive reversible inhibitor.

Lineweaver–Burk plot, which is a graph that allows you to analyse enzyme kinetics derived from Michaelis-Menten equation, can be used.

The Michaelis–Menten equation describes the kinetic behaviour of enzyme-catalyzed reactions. It is a mathematical formula that determines the ratio of reaction rate to substrate concentration. Lineweaver Burk is a plot that shows the double reciprocal of the Michaelis–Menten equation. It is also a linearization. The plot shows the rate at which the reaction occurs (y-axis), and the inverse of substrate concentration (1/).[S], x-axis). The Lineweaver Burk plot is useful for analyzing the impact of an inhibitor that can compete with the reversible. This plot will be a straight-line with the yintercept equal to Vmax and the intercept equal to Ki (Rajagopalan, Subramanian 2016). Vmax is reduced by the inhibitor, so the y-intercept in the is less than the. Cont…